What is the macromolecular composition of the cell?. What are the concentrations of cytoskeletal molecules?. How much cell-to-cell variability exists in protein expression?. What are the most abundant proteins in a cell?. What lipids are most abundant in membranes?. What are the concentrations of free metabolites in cells?. What are the concentrations of different ions in cells?. What is the concentration of bacterial cells in a saturated culture?. What quantities of nutrients need to be supplied in growth media?. What are environmental O2 and CO2 concentrations?. What is the elemental composition of a cell?. Concentrations and Absolute Numbers – Introduction. II: Concentrations and Absolute Numbers. What is the thickness of the cell membrane?. 
How big are the molecular machines of the central dogma?. Which is bigger, mRNA or the protein it codes for?. 
How big are biochemical nuts and bolts?.How big is the endoplasmic reticulum of cells?.
What is the range of cell sizes and shapes?.How to make back-of-the-envelope calculations.
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